The Little River Spring pool measures 108 ft north to south and 93 ft east to west. The depth over the vent is 11.0 ft. Limestone is exposed in the pool, and there are areas covered by sand. Spring water issues from an elongated fracture in the limestone. The water is clear and greenish blue. Algae cover approximately half the area in the spring and run, and frequent swimming probably keeps the other half cleared. There is a sizeable boil over the vent near the center of the spring pool.
The spring discharge flows through a 150 ft long run southwesterly into the Suwannee River from the east. Land surrounding the spring is generally forested. An extensive cave system exists below this spring. Cave divers report that the cave system reaches depths of well over 100 ft.
Little River Spring is located 3.5 miles north of Branford. From the intersection of US 27 and US 129 in Branford, travel north on US 129 for 3.1 miles. Turn west (left) on CR 248 and travel 1.7 miles. The spring is located on the south (left) side of the road.